Using PepExplorer to Filter and Organize De Novo Peptide Sequencing Results

Published in Current Protocols in Bioinformatics, 2015

Recommended citation: da Veiga Leprevost F, Barbosa VC, Carvalho PC. Using PepExplorer to Filter and Organize De Novo Peptide Sequencing Results. Curr Protoc Bioinformatics. 2015;51:13.27.1-13.27.9. Published 2015 Sep 3. doi:10.1002/0471250953.bi1327s51 https://pubmed.ncbi.nlm.nih.gov/26334921/

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PepExplorer aids in the biological interpretation of de novo sequencing results; this is accomplished by assembling a list of homolog proteins obtained by aligning results from widely adopted de novo sequencing tools against a target-decoy sequence database. Our tool relies on pattern recognition to ensure that the results satisfy a user-given false-discovery rate (FDR). For this, it employs a radial basis function neural network that considers the precursor charge states, de novo sequencing scores, the peptide lengths, and alignment scores. PepExplorer is recommended for studies addressing organisms with no genomic sequence available. PepExplorer is integrated into the PatternLab for proteomics environment, which makes available various tools for downstream data analysis, including the resources for quantitative and differential proteomics.

Recommended citation: da Veiga Leprevost F, Barbosa VC, Carvalho PC. Using PepExplorer to Filter and Organize De Novo Peptide Sequencing Results. Curr Protoc Bioinformatics. 2015;51:13.27.1-13.27.9. Published 2015 Sep 3. doi:10.1002/0471250953.bi1327s51