A data processing toolkit for shotgun proteomics.
Philosopher provides easy access to third-party tools and custom algorithms allowing users to develop proteomics analysis, from Peptide Spectrum Matching to annotated protein reports. Philosopher is also tunned for Open Search analysis, providing a modified version of the prophets for peptide validation and protein inference. To this date, Philosopher is the only proteomics toolkit that allows you to process and analyze close and open search results.
Database downloading and formatting.
Peptide Spectrum Matching with Comet.
Peptide assignment validation with PeptideProphet.
Multi-level integrative analysis with iProphet.
PTM site localization with PTMProphet.
Protein inference with ProteinProphet.
Open Search data validation.
FDR filtering with custom algorithms.
- Two-dimensional filtering for simultaneous control of PSM and Protein FDR levels.
- Sequential FDR estimantion for large data sets using filtered PSM and proteins lists.
- PickedFDR for scalable estimations.
- Razor peptides determination for better quantification and interpretationinterpretation.
Label-free quantification via Spectral counting and MS1 Quantification.
Labeling-based quantification using TMT isobaric tags.
Clustering analysis for proteomics results.
Multi-level detailed reports including peptides, ions and proteins.
How to Download
Download the latest version here
How to Use
A simple tutorial is also provided with an extensive example on how to use Philosopher.
Check the documentation for more details about the available commands.
Questions, requests and bug reports
If you have any questions, remarks, requests or if you found a bug, please use the Issue tracker.
How to cite
Still in progress!
About the developers
Another great work from Alexey Nesvizhskii’s nesvilab